![]() ![]() ![]() If the medium is sterilized, it can be stored in the refrigerator until use. Some laboratories expose the medium for 10 minutes in fluent steam to sterilize it, since an autoclave should not be used. It can be heated slightly to dissolve completely. If you have the components of the mixture, you can weigh:Ĥ g of anhydrous sodium hydrogen selenite.ĭissolve the compounds in 1 liter of sterile distilled water. There is also a variant to which cystine is added, hence its name selenite-cystine broth. It is composed of anhydrous sodium hydrogen selenite, anhydrous sodium phosphate, peptones, and lactose. However, it also allows the recovery of Shigella strains and does not inhibit the growth of Pseudomonas and Proteus. It is primarily toxic to most bacteria belonging to the Enterobacteriaceae Family. Its chemical composition favors the recovery of these microorganisms and in turn inhibits the growth of others. This medium complies with the requirements of the American Public Health Association (APHA), and therefore is accepted for the investigation of the presence of Salmonella in samples of stool, urine, liquid or solid food, water, among others. It was designed by Leifson for the enrichment of samples where the presence of enteropathogenic bacteria of the genus Salmonella is suspected. Neufeld F (1902) Über die Agglutination der Pneumokokken und über die Theorien der Agglutination.The selenite broth It is a selective liquid culture medium. American Society for Microbiology, Washington, DC ![]() Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH (1999) Manual of clinical microbiology, 7th edn. Am J Public Health Nations Health 39:866–869 McCullough NB (1949) Laboratory tests in the diagnosis of brucellosis. Hewitt LF, Todd EW (1932) A new culture medium for the production of antigenic streptococcal hæmolysin. Van De Rijn I, Kessler E (1980) Growth characteristics of group a streptococci in a new chemically defined medium. Texeira E, Checa J, Rial A, Chabalgoity JA, Suárez N (2015) A new chemically defined medium for cultivation of Streptococcus pneumoniae serotype 1. ![]() Russell FM, Biribo SSN, Selvaraj G, Oppedisano F, Warren S, Seduadua A, Mulholland EK, Carapetis JR (2006) As a bacterial culture medium, citrated sheep blood agar is a practical alternative to citrated human blood agar in Laboratories of Developing Countries. Luria SE, Burrous JW (1957) Hybridization between Escherichia Coli and Shigella. Hoeprich PO (1957) Evaluation of an improved chemically defined medium for the culture of Diplococcus pneumoniae. Suárez N (2016) Optimal conditions for Streptococcus pneumoniae culture and for polysaccharide production for vaccines. Suárez N, Franco Fraguas L, Ferreira F, Massaldi M (2008) Improved conjugation and purification strategies for the preparation of protein polysaccharide conjugates. Tarahomjoo S, Jalali M (2015) Investigation of appropriate cultivation approach for capsular polysaccharide production by Streptococcus pneumoniae serotype 19 American journal of. Leal MM, Pereira DSG, Jessouroun E, Couto MAPG, Pereira N (2001) Investigation of cultivation conditions for capsular polysaccharide production by Streptococcus pneumoniae serotype 14. Massaldi H, Besssio MI, Suarez N, Texeira E, Rossi S, Ferreira F (2010) Features of bacterial growth and polysaccharide production of Streptococcus pneumoniae serotype 14. Kim SN, Min KK, Choi HI, Kim SW, Pio SN, Rhee DK (1996) Optimization of culture conditions for production of pneumococcal capsular polysacharide type IV. (1980) Procédé de purification de polyosides de Streptococcus pneumoniae et vaccin à base de polyosides ainsi purifiés Brevet Belge No. ![]()
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